Product name: Human Amylase ELISA Kit

Description:
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Amylase. Standards or samples are added to the appropriate micro ELISA plate wells and combined to the specific antibody. Then a biotinylated detection antibody specific for Amylase and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Amylase, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turn yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Amylase.You can calculate the concentration of Amylase in the samples by comparing the OD of the samples to the standard curve.