Product name:Human Vascular Endothelial cell Growth Factor C,VEGF-C ELISA KIT

Description:
Intended use
This immunoassay kit allows for the specific measurement of human VEGF-C concentrations in cell culture supernates, serum, and plasma.
 
Summary
Vascular endothelial growth factor-C (VEGF-C) is a member of the VEGF family of cytokines. It has been shown to exhibit angiogenic and lymphangiogenic actions. The VEGF family of growth factors and receptors is involved in the development and growth of the vascular endothelial system. Two of its family members, VEGF-C and VEGF-D, regulate the lymphatic endothelial cells via their receptor VEGFR, thus acting as mitogens for these cells. VEGF-C expression is associated with hematological malignancies. Like VEGF it acts as survival factor on leukemia. Together with the expression of their receptors, VEGF and VEGF-C result in the generation of autocrine loops that may support cancer cell survival and proliferation. Further VEGF-C expression has been shown in gastrointestinal tract malignancies where it correlates with lymphatic invasion, lymphnode metastasis and reduced survival.
 
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for VEGF-C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF-C present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for VEGF-C is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF-C bound in the initial step. The color development is stopped and the intensity of the color is measured.